Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 3: Changes of p-ATM, p53, p21, p-Chk1, p-Chk2 and p-cdc25C protein expression levels in the kidney at 42 days of age. (Immunohistochemistry, ×400).

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Immunohistochemistry

Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 5: Changes of the mean density of p-ATM, p53, p21, p-Chk1, p-Chk2 and p-cdc25C protein expression in the kidney. Data are presented with the mean ± standard deviation (n=5×5) *P<0.05, compared with the control group **P<0.01, compared with the control group.

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Standard Deviation, Control

Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 7: Changes of ATM, p53, p21, Chk1, Chk2 and cdc25 mRNA expression levels in the kidney. Data are presented with the mean ± standard deviation (n=5) *P<0.05, compared with the control group **P<0.01, compared with the control group.

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and RAC1/CDC42 alterations in rats

doi: 10.1371/journal.pone.0185102

Figure Lengend Snippet: ( A ) Western blot bands of the hippocampal tissues determined with RAC1, CDC42, and RHOA antibodies in CG (left column), DA (middle left column), Pa2 (middle right column), and Pa6 (right column). GADPH (36KD) is an internal reference. Pre-treatments with Pa (50 mg/kg, i.p.) for 6 weeks significantly alleviated D -gal and AlCl 3 -induced upregulation of RAC1 ( B ) and CDC42 ( C ); however, it did not significantly reduce the expression of RHOA ( D ). Treatment with Pa for 2 weeks also significantly alleviated D -gal and AlCl 3 -induced increase of CDC42. Data expressed as the means ± SEM (n = 3~5). ** P< 0.01, *** P< 0.001, DA versus CG; # P< 0.05, ### P< 0.001, Pa2 versus CG; ΦΦ P< 0.01, ΦΦΦ P< 0.001, Pa6 versus CG; Δ P< 0.05, DA versus Pa2; $$ P< 0.01, $$$ P< 0.01, DA versus Pa6; && P< 0.01, Pa2 versus Pa6.

Article Snippet: CDC42 and RAC1 antibodies were purchased from Boster Biological Technology Co., LTD (Wuhan, China).

Techniques: Western Blot, Expressing

Journal: Oncotarget

Article Title: miR-320a functions as a suppressor for gliomas by targeting SND1 and β-catenin, and predicts the prognosis of patients

doi: 10.18632/oncotarget.14975

Figure Lengend Snippet: A . Growth curves from U87MG and U251 cells transfected with Scr or miR-320a and cotransfected with miR-320a plus plasmid expressing β-catenin (miR-320a+CTNNB1) or SND1 (miR-320a+SND1) assessed by CCK8 assay. B and C . EdU-positive rate (B) and representative images (C) in the indicated cells assessed by EdU assay. D and E . Representative images (left) and percentage of each phase cells (right) in the indicated cells assessed by FCM. F and G . Western blot analyses of β-catenin, cyclin D1, SND1 and p21 WAF1 (left), and comparisons among groups of their expressions (right) in the cells as indicated. Their relative expression levels were normalized against β-actin. All the experiments were performed at least in triplicate and the data are presented as the mean ± SD. * P <0.05, ** P <0.01, ***/ ▲▲▲ P <0.001. Compared with Scr group* and with miR-320a group ▲ in FCM data.

Article Snippet: Rabbit anti-human β-catenin, MMP7, cyclin D1, Smad2 and phosphorylated Smad2 (CST), mouse anti-human SND1 (Abcam), Smad4 (R&D Systems, Minneapolis, MN, USA), MMP2, p21 WAF1 and β-actin (Boster Biological Technology, Wuhan, China) were used as primary antibodies.

Techniques: Transfection, Plasmid Preparation, Expressing, CCK-8 Assay, EdU Assay, Western Blot

Journal: Cancer Cell International

Article Title: Mesenchymal stem cells and cancer stem cells promote colorectal cancer cell growth and biological functions via cell–cell indirect contact

doi: 10.1186/s12935-025-03993-7

Figure Lengend Snippet: Indirect co-culture of MSCs regulates the CRC cell cycle. (A) CRC cell cycle distribution as assessed using flow cytometry. (B) Immunoblotting assessment of P16, P21 and P53 levels, with GAPDH as the loading control. * P < 0.05 compared with the control and ** P < 0.01 compared with the. control

Article Snippet: The primary antibodies recognized: CD133 (ABclonal, Wuhan, China; No. A0818, 1:1000), CD44 (ABclonal; No. A12410, 1:1000), OCT4 (Beyotime; No. AF2506, 1:1000), SOX2 (Beyotime; No. AF8034, 1:1000), NANOG (Beyotime; No. AF1912, 1:1000), E-cadherin (Cell Signaling Technology (CST), Danvers, MA, USA; No. 3195, 1:1000), N-cadherin (CST; No. 13116, 1:1000), Vimentin (CST; No. 5741, 1:1000), Caspase-3 (Beyotime, No. AF0081, 1:1000), BCL2 associated X protein (Bax) (Beyotime; No. AF1270, 1:1000), B-cell CLL/lymphoma 2 (Bcl-2) (Beyotime; No. AF6285, 1:1000), tumor protein 53 (P53) (Boster; No. BM4026, 1:1000), P21 (Proteintech, Rosemont, IL, USA; No. 10355-1-AP, 1:1000), P16 (Beyotime, No. AF1672, 1:1000), PI3K (Beyotime; No. AF1966, 1:1000), phosphorylated (p)-PI3K (Beyotime; No. AF1966, 1:1000), AKT (Beyotime; No. AF1777, 1:1000), p-AKT (Beyotime; No. AA329, 1:1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime; No. AF0006, 1:1000).

Techniques: Co-Culture Assay, Flow Cytometry, Western Blot, Control

Journal: Journal of animal science and biotechnology

Article Title: CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.

doi: 10.1186/s40104-023-00884-7

Figure Lengend Snippet: Fig. 3 CLOCK interference promotes GCs proliferation. A The interference efficiency of CLOCK was measured using RT-qPCR. Data are expressed as mean ± SEM (n = 5), **P < 0.01. B Western blotting reveals the expression levels of CLOCK. C Quantification of the western blot analysis. Data are expressed as mean ± SEM (n = 3), *P < 0.05. D EdU staining was used to detect the number of proliferating cells. RED, EdU-positive cells; BLUE, Hoechst staining for total nuclei. Data are expressed as mean ± SEM (n = 5), **P < 0.01. E CCK-8 assay detecting cell viability at 24 h after transfection. Data are expressed as mean ± SEM (n = 5), *P < 0.05. F RT-qPCR analysis of proliferation-related genes, including CCNB1, CCND1, CCNE1, CDK1, and CDK4. Data are expressed as mean ± SEM (n = 5), *P < 0.05, **P < 0.01. G Western blot analysis of proliferation-related gene protein level (CLOCK, CCNB1, CCNE1, CDK4, and CDKN1A). GAPDH as a housekeeping protein. H Quantifying the Western blot analysis of CLOCK, CCNB1, CCNE1, CDK4, and CDKN1A. Data are expressed as mean ± SEM (n = 3), *P < 0.05

Article Snippet: Table 1 The information of antibodies Reagent type Designation Source Catalog No. Dilution rate/concentration Antibody GAPDH Abways AB0036 WB(1:5,000) Antibody CLOCK Abways CY6972 WB(1:1,000), IF(1:100) Antibody CCNB1 Abways CY5378 WB(1:1,000) Antibody CCND1 Abways CY5404 WB(1:1,000) Antibody CCNE1 Abways CY1028 WB(1:1,000) Antibody CDK4 Abways CY5836 WB(1:1,000) Antibody CDKN1A Abways CY5088 WB(1:1,000) Antibody ASB9 Santa Cruz sc-166723 WB(1:1,000) Antibody HRP conjugated AffiniPure goat anti-mouse IgG (H + L) Boster BA1051 WB(1:5,000) Antibody HRP conjugated AffiniPure goat anti-rabbit IgG (H + L) Boster BA1054 WB(1:5,000) Antibody CY3 conjugated AffiniPure goat anti-rabbit IgG (H + L) Boster BA1032 IF(1:100) Antibody Anti-mouse IgG goat monoclonal antibody Boster M04575-3 ChIP (1μg) Antibody CLOCK Santa Cruz sc-271603 ChIP (1μg)

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, CCK-8 Assay, Transfection

Journal: Journal of animal science and biotechnology

Article Title: CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.

doi: 10.1186/s40104-023-00884-7

Figure Lengend Snippet: Fig. 6 ASB9 interference promotes GCs proliferation. A RT-qPCR detected the interference efficiency of ASB9. Data are expressed as mean ± SEM (n = 6), **P < 0.01. B Western blotting reveals the expression levels of ASB9. C Quantification of the western blot analysis. Data are expressed as mean ± SEM (n = 3), *P < 0.05. D EdU staining was used to detect the number of proliferating cells. RED, EdU-positive cells; BLUE, Hoechst staining for total nuclei. Data are expressed as mean ± SEM (n = 4), **P < 0.01. E CCK-8 assay detecting cell viability at 24 h after transfection. Data are expressed as mean ± SEM (n = 16), ****P < 0.0001. F RT-qPCR analysis of proliferation-related genes, including CCNB1, CCND1, CCNE1, CDK1, and CDK4. Data are expressed as mean ± SEM (n = 5), *P < 0.05, **P < 0.01. G Western blot analysis of proliferation-related gene protein level (ASB9, CCNB1, CCNE1, CDK4, and CDKN1A). H Quantifying the western blot analysis of CLOCK, CCNB1, CCNE1, CDK4, and CDKN1A. Data are expressed as mean ± SEM (n = 3), *P < 0.05, **P < 0.01. I RNA expression of ASB9 in GCs. ZT: zone time

Article Snippet: Table 1 The information of antibodies Reagent type Designation Source Catalog No. Dilution rate/concentration Antibody GAPDH Abways AB0036 WB(1:5,000) Antibody CLOCK Abways CY6972 WB(1:1,000), IF(1:100) Antibody CCNB1 Abways CY5378 WB(1:1,000) Antibody CCND1 Abways CY5404 WB(1:1,000) Antibody CCNE1 Abways CY1028 WB(1:1,000) Antibody CDK4 Abways CY5836 WB(1:1,000) Antibody CDKN1A Abways CY5088 WB(1:1,000) Antibody ASB9 Santa Cruz sc-166723 WB(1:1,000) Antibody HRP conjugated AffiniPure goat anti-mouse IgG (H + L) Boster BA1051 WB(1:5,000) Antibody HRP conjugated AffiniPure goat anti-rabbit IgG (H + L) Boster BA1054 WB(1:5,000) Antibody CY3 conjugated AffiniPure goat anti-rabbit IgG (H + L) Boster BA1032 IF(1:100) Antibody Anti-mouse IgG goat monoclonal antibody Boster M04575-3 ChIP (1μg) Antibody CLOCK Santa Cruz sc-271603 ChIP (1μg)

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, CCK-8 Assay, Transfection, RNA Expression